Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii
نویسندگان
چکیده
منابع مشابه
Chromatin immunoprecipitation (ChIP).
Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with post-translational modifications at specific genomic l...
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Epigenetics remains a rapidly developing field that studies how the chromatin state contributes to differential gene expression in distinct cell types at different developmental stages. Epigenetic regulation contributes to a broad spectrum of biological processes, including cellular differentiation during embryonic development and homeostasis in adulthood. A critical strategy in epigenetic stud...
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Chromatin immunoprecipitation (ChIP) has become a central method when studying in vivo protein-DNA interactions, with the major challenge being the hope to capture "authentic" interactions. While ChIP protocols have been optimized for use with specific cell types and tissues including adipose tissue-derived cells, a working ChIP protocol addressing the challenges imposed by fresh whole human ad...
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Signaling pathways regulate gene expression programs via the modulation of the chromatin structure at different levels, such as by post-translational modifications (PTMs) of histone tails, the exchange of canonical histones with histone variants, and nucleosome eviction. Such regulation requires the binding of signal-sensitive transcription factors (TFs) that recruit chromatin-modifying enzymes...
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SoliD System chip-Seq Analysis The SOLiD System’s ability to generate over 400 million sequence tags (35 bp sequence reads) in a single run enables whole genome ChIP analysis of complex organisms. Sequence tags are mapped to a reference sequence and counted, to identify specific regions of protein binding. The ultra high throughput of the system provides researchers with the sensitivity and the...
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ژورنال
عنوان ژورنال: Plant Methods
سال: 2011
ISSN: 1746-4811
DOI: 10.1186/1746-4811-7-35